KomivesLab

Specializing in Biophysics Research

Mass Spectrometry

Lumpkin RJ, Gu H, Zhu Y, et al. Site-specific identification and quantitation of endogenous SUMO modifications under native conditions. (2017) Nat Commun. 2017;8(1):1171.

Ramírez-Sarmiento CA, Baez M, Zamora RA, Balasubramaniam D, Babul J, Komives EA, Guixé V. (2015) The folding unit of phosphofructokinase-2 as defined by the biophysical properties of a monomeric mutant. Biophys J 108(9):2350-61

Meyer JG, Kim S, Maltby DA, Ghassemian M, Bandeira N, Komives EA. (2014) Expanding proteome coverage with orthogonal-specificity α-lytic proteases. Mol Cell Proteomics 13(3):823-35.

Ramirez-Sarmiento C, Baez M, Wilson CAM, Babul J, Komives EA, Guixé V. (2013) Observation of solvent penetration during cold denaturation of E. coli phosphofructokinase-2. Biophys J 104, 2254-2263.

Balasubramaniam D, Komives EA. (2012) Hydrogen-exchange mass spectrometry for the study of intrinsic disorder in proteins. Biochim Biophys Acta. doi:pii: S1570-9639(12)00250-6. 10.1016/j.bbapap.2012.10.009. PMC3600394

Meyer JG, Komives EA. (2012) Charge state coalescence during electrospray ionization improves peptide identification by tandem mass spectrometry. J Am Soc Mass Spec 23, 1390-1399. doi: 10.1007/s13361-012-0404-0.

Craig PO, Lätzer J, Weinkam P, Hoffman RM, Ferreiro DU, Komives EA, Wolynes PG. (2011) Prediction of native-state hydrogen exchange from perfectly funneled energy landscapes. J Am Chem Soc. 133(43):17463-72. PMC3203634

Gertsman, I., Fu, C., Huang, R., Komives, E. A., Johnson, J. E. (2010) Critical salt bridges guide capsid assembly, stability, and maturation behavior in Bacteriophage HK97. Mol Cell Proteomics 9, 1752-63. PMC2938056.

Gertsman, I., Komives, E. A., Johnson, J. E. (2010) HK97 Maturation Studied by Crystallography and H/(2)H Exchange Reveals the Structural Basis for Exothermic Particle Transitions. J Mol Biol. 397, 560-574. PMC2938056.

Gertsman, I., Gan, L., Guttman, M., Lee, K., Duda, R. L., Hendrix, R. W., Komives, E. A., Johnson, J. E. (2009)An unexpected twist in viral capsid maturation Nature 458, 646-50.PMC2765791

Anand, G. S., Hotchko, M., Brown, S. H., Ten Eyck, L. F., Komives, E. A. and Taylor, S. S. (2007) R-subunit Isoform Specificity in Protein Kinase A: Distinct Features of Protein Interfaces in PKA Types I and II by Amide H/(2)H Exchange Mass Spectrometry. J Mol Biol. 374, 487-99.

Truhlar, S. M. E., Croy, C. H., Torpey, J. W., Koeppe, J. R. & Komives, E. A. (2006) Solvent Accessibility of Protein Surfaces by Amide H/2H Exchange MALDI-TOF Mass Spectrometry. J. Am. Soc. Mass Spectrom. 17, 1490 – 97.

Hotchko, M., Anand, G., Komives, E. A., & Ten Eyck, L. (2006) Automated extraction of backbone deuteration levels from amide H/2H exchange mass spectrometry experiments Protein Science 15, 583 – 601.

Komives, E. A.(2005) Protein-protein interaction dynamics by amide H/2H exchange mass spectrometryInternational Journal of Mass Spectrometry 240, 285-290.

Anand, G. S., Law, D., Mandell, J. G., Snead, A. N., Tsigelny, I., Taylor, S. S., Ten Eyck, L., & Komives, E. A. (2003) Identification of the Protein Kinase A Regulatory RI -Catalytic Subunit Interface by Amide H/2H Exchange and Protein Docking Proc. Nat. Acad. Sci. U. S. A. 100, 13264-13269.

Anand, G. S., Hughes, C. A., Jones, J. M., Taylor, S. S. & Komives, E. A. (2002) Amide H/2H exchange reveals communication between the cAMP- and catalytic subunit-binding sites in the regulatory subunit of protein kinase A. J. Mol. Biol. 323, 377-386.

Baerga-Ortiz, A; Hughes, CA; Mandell, JG; Komives, EA. (2002) Epitope mapping of a monoclonal antibody against human thrombin by H/D exchange mass spectrometry reveals selection of a diverse sequence in a highly conserved protein. Protein Sci 11, 1300-1308.

Hughes, C. A., Mandell, J. G., Anand, G. S., Stock, A. M. & Komives, E. A. (2001) Phosphorylation Causes Subtle Changes in Solvent Accessibility at the Interdomain Interface of Methylesterase CheB J. Mol. Biol. 307, 967-976.

Mandell, JG; Baerga-Ortiz, Komives, EA.(2001) Solvent accessibility of the thrombin-thrombomodulin interface.J. Mol. Biol. 306: 575-589.

Mandell, JG; Falick, AM; Komives, EA. Identification of protein-protein interfaces by decreased amide proton solvent accessibility. Proceedings of the National Academy of Sciences of the United States of America, 1998 Dec 8, 95(25):14705-10.

Mandell, JG; Falick, AM; Komives, EA. Measurement of amide hydrogen exchange by MALDI-TOF mass spectrometry. Analytical Chemistry, 1998 Oct 1, 70(19):3987-95.